African Evaluation Database

7507

Source

Scopus

Electronic ID

2-s2.0-79959522651

Authors

Kagulire S.C., Opendi P., Stamper P.D., Nakavuma J.L., Mills L.A., Makumbi F., Gray R.H., Shott J.P., Serwadda D., Reynolds S.J.

Title

Field evaluation of five rapid diagnostic tests for screening of HIV-1 infections in rural Rakai, Uganda

Publication Year

2011

Source Title

International Journal of STD and AIDS

Volume

Issue

Citations

DOI

10.1258/ijsa.2009.009352

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-79959522651&partnerID=40&md5=2a6b2737804277b0c1e3d475ec819f30

Affiliations

Rakai Health Sciences Program, Kalisizo, Uganda; School of Medicine, Johns Hopkins University, Baltimore, MD, United States; Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda; School of Public Health, Makerere University, Kampala, Uganda; Clinical Monitoring Research Program, SAIC-Frederick, Inc, NCI-Frederick, Frederick, MD, United States; National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States

Authors with Affiliations

Kagulire, S.C., Rakai Health Sciences Program, Kalisizo, Uganda; Opendi, P., Rakai Health Sciences Program, Kalisizo, Uganda; Stamper, P.D., School of Medicine, Johns Hopkins University, Baltimore, MD, United States, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; Nakavuma, J.L., Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda; Mills, L.A., Rakai Health Sciences Program, Kalisizo, Uganda, School of Medicine, Johns Hopkins University, Baltimore, MD, United States; Makumbi, F., Rakai Health Sciences Program, Kalisizo, Uganda, School of Public Health, Makerere University, Kampala, Uganda; Gray, R.H., Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States; Shott, J.P., Clinical Monitoring Research Program, SAIC-Frederick, Inc, NCI-Frederick, Frederick, MD, United States, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States; Serwadda, D., School of Public Health, Makerere University, Kampala, Uganda; Reynolds, S.J., National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States

Abstract

The performance characteristics of HIV rapid diagnostic tests (RDTs) vary by test and by population. We assessed five commercial RDTs in Uganda where all but one RDT (Determine; Abbott Laboratories, Germany) performed close to manufacturer's expectations. Determine had low specificity (85.2%, positive predictive value 67.3%) due to false-positive results with weakpositive bands. Properly trained staff, good quality control programmes and validation of RDTs with laboratories having confirmatory testing capacity may be warranted to assure accuracy in each setting.

Author Keywords

HIV; Point of care tests; Rapid diagnostic testing; Resource-poor settings; Uganda

Index Keywords

Advanced Quality Rapid Anti HIV 1 2 test; article; clinical evaluation; Determine HIV 1 2 test; diagnostic accuracy; false positive result; First Response 1 2.0 test; HIV test; human; Human immunodeficiency virus 1 infection; laboratory personnel; major clinical study; predictive value; priority journal; quality control; rapid diagnostic test; screening test; sensitivity and specificity; STAT PAK HIV 1 2 test; Uganda; Uni Gold HIV 1 2 test; validation process; Blotting, Western; Cohort Studies; Enzyme-Linked Immunosorbent Assay; False Positive Reactions; HIV Infections; HIV-1; Humans; Point-of-Care Systems; Reagent Kits, Diagnostic; Reproducibility of Results; Rural Population; Sensitivity and Specificity; Uganda

Funding Details

None