de Beer D., Jerz G., Joubert E., Wray V., Winterhalter P.
Post-Harvest and Wine Technology Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa; Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, D-38106 Braunschweig, Germany; Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602 Stellenbosch, South Africa; Department of Structural Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany
de Beer, D., Post-Harvest and Wine Technology Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa; Jerz, G., Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, D-38106 Braunschweig, Germany; Joubert, E., Post-Harvest and Wine Technology Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa, Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602 Stellenbosch, South Africa; Wray, V., Department of Structural Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany; Winterhalter, P., Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, D-38106 Braunschweig, Germany
Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. © 2009 Elsevier B.V. All rights reserved.
Counter-current chromatography; Cyclopia subternata; Fabaceae; Isomangiferin; Mangiferin; NMR spectroscopy; Xanthones; Acetonitrile; Body fluids; Bubbles (in fluids); Chromatographic analysis; Chromatography; Electrospray ionization; Esters; Ethers; High performance liquid chromatography; High pressure liquid chromatography; Liquids; Methanol; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Organic solvents; Phenols; Solvent extraction; acetic acid ethyl ester; coumaric acid; eriocitrin; hesperidin; isomangiferin; luteolin; mangiferin; methanol; phenol derivative; scolymoside; unclassified drug; water; article; carbon nuclear magnetic resonance; counter current chromatography; Cyclopia subternata; drug isolation; drug structure; electrospray mass spectrometry; extraction; heteronuclear multiple bond correlation; high performance liquid chromatography; medicinal plant; nonhuman; plant leaf; priority journal; proton nuclear magnetic resonance; Chromatography, High Pressure Liquid; Countercurrent Distribution; Cyclopia Plant; Methanol; Nuclear Magnetic Resonance, Biomolecular; Plant Extracts; Xanthones; Cyclopia subternata; Fabaceae
