Awolola S.T., Adeogun A.O., Olojede J.B., Oduola A.O., Oyewole I.O., Amajoh C.N.
Molecular Entomology and Vector Control Research Laboratory, Nigerian Institute of Medical Research, PMB 2013 Yaba Lagos, Nigeria; Department of Zoology, University of Ibadan, Ibadan, Nigeria; Department of Zoology, University of Ilorin, Ilorin, Nigeria;
Awolola, S.T., Molecular Entomology and Vector Control Research Laboratory, Nigerian Institute of Medical Research, PMB 2013 Yaba Lagos, Nigeria; Adeogun, A.O., Molecular Entomology and Vector Control Research Laboratory, Nigerian Institute of Medical Research, PMB 2013 Yaba Lagos, Nigeria, Department of Zoology, University of Ibadan, Ibadan, Nigeria; Olojede, J.B., Molecular Entomology and Vector Control Research Laboratory, Nigerian Institute of Medical Research, PMB 2013 Yaba Lagos, Nigeria; Oduola, A.O., Department of Zoology, University of Ilorin, Ilorin, Nigeria; Oyewole, I.O., Department of Biological Sciences, Babcock University, Ilishan Remo, Nigeria; Amajoh, C.N., National Malaria Control Program, Federal Ministry of Health, Abuja, Nigeria
Background: PermaNet® 3.0 is an insecticide synergist-combination long-lasting insecticidal net designed to have increased efficacy against malaria vectors with metabolic resistance, even when combined with kdr. The current study reports on the impact of this improved tool on entomological indices in an area with pyrethroid-resistant malaria vectors in Nigeria. Methods. Baseline entomological indices across eight villages in Remo North LGA of Ogun State provided the basis for selection of three villages (Ilara, Irolu and Ijesa) for comparing the efficacy of PermaNet® 3.0 (PN3.0), PermaNet® 2.0 (PN2.0) and untreated polyester nets as a control (UTC). In each case, nets were distributed to cover all sleeping spaces and were evaluated for insecticidal activity on a 3-monthly basis. Collection of mosquitoes was conducted monthly via window traps and indoor resting catches. The arithmetic means of mosquito catches per house, entomological inoculation rates before and during the intervention were compared as well as three other outcome parameters: the mean mosquito blood feeding rate, mean mortality and mean parity rates. Results: Anopheles gambiae s.l. was the main malaria vector in the three villages, accounting for >98% of the Anopheles population and found in appreciable numbers for 6-7 months. Deltamethrin, permethrin and lambdacyhalothrin resistance were confirmed at Ilara, Irolu and Ijesa. The kdr mutation was the sole resistance mechanism at Ilara, whereas kdr plus P450-based metabolic mechanisms were detected at Irolu and Ijesa. Bioassays repeated on domestically used PN 2.0 and PN 3.0 showed persistent optimal (100%) bio-efficacy for both net types after the 3rd, 6th, 9 th and 12th month following net distribution. The use of PN 3.0 significantly reduced mosquito densities with a 'mass killing' effect inside houses. Households with PN 3.0 also showed reduced blood feeding as well as lower mosquito parity and sporozoite rates compared to the PN 2.0 and the UTC villages. A significant reduction in the entomological inoculation rate was detected in both the PN 2.0 village (75%) and PN 3.0 village (97%) post LLIN-distribution and not in the UTC village. Conclusion: The study confirms the efficacy of PN 3.0 in reducing malaria transmission compared to pyrethroid-only LLINs in the presence of malaria vectors with P450-based metabolic- resistance mechanisms. © 2014 Awolola et al.; licensee BioMed Central Ltd.
cyhalothrin; cytochrome P450; deltamethrin; permethrin; polyester; pyrethroid; vasculotropin receptor 2; insect protein; insecticide; pyrethroid; adult; Anopheles arabiensis; Anopheles funestus; Anopheles gambiae; anopheles nili; arithmetic; article; bed net; bioassay; blood; blood feeding; comparative effectiveness; controlled study; density; feeding; gene; gene mutation; household; human; inoculation; insecticidal activity; insecticide resistance; kdr gene; malaria; mortality; multicenter study; Nigeria; nonhuman; parity; Plasmodium falciparum; randomized controlled trial; reduction; sleep; space; sporozoite; vector control; animal; Anopheles gambiae; disease carrier; drug effects; family size; feeding behavior; gene expression regulation; genetics; information processing; malaria; metabolism; mutation; population density; questionnaire; time; Anopheles gambiae; Animals; Anopheles gambiae; Data Collection; Family Characteristics; Feeding Behavior; Gene Expression Regulation; Insect Proteins; Insect Vectors; Insecticide Resistance; Insecticide-Treated Bednets; Insecticides; Malaria; Mutation; Nigeria; Population Density; Pyrethrins; Questionnaires; Time Factors
