Ng'ayo M.O., Friedrich D., Holmes K.K., Bukusi E., Morrow R.A.
Kenya Medical Research Institute, Lumumba Health Center, CMR-UCSF Building, Box 614 - 40100, Kisumu, Kenya; Seattle Children's, MailstopW8814, Seattle, WA 98105 - 0371, United States; Center for AIDS, STD, University of Washington, Box 356355 Seattle, WA 98195 - 6355, United States; Department of Obstetrics and Gynecology, University of Nairobi, Box 1976 - 00202, Nairobi, Kenya; Department of Laboratory Medicine, University of Washington, Box 358080, Seattle, WA 98109-1024, United States
Ng'ayo, M.O., Kenya Medical Research Institute, Lumumba Health Center, CMR-UCSF Building, Box 614 - 40100, Kisumu, Kenya; Friedrich, D., Seattle Children's, MailstopW8814, Seattle, WA 98105 - 0371, United States; Holmes, K.K., Center for AIDS, STD, University of Washington, Box 356355 Seattle, WA 98195 - 6355, United States; Bukusi, E., Kenya Medical Research Institute, Lumumba Health Center, CMR-UCSF Building, Box 614 - 40100, Kisumu, Kenya, Department of Obstetrics and Gynecology, University of Nairobi, Box 1976 - 00202, Nairobi, Kenya; Morrow, R.A., Seattle Children's, MailstopW8814, Seattle, WA 98105 - 0371, United States, Department of Laboratory Medicine, University of Washington, Box 358080, Seattle, WA 98109-1024, United States
This study compared five serological tests with Western blot from University of Washington to determine the most accurate method for detecting antibodies to herpes simplex virus type 2 (HSV-2) in a male population in Kisumu, Kenya. A random sample of 250 fishermen from 18 beaches along Lake Victoria underwent serological testing by two generations of the HerpeSelect HSV-2 ELISA ("Focus Gen 1" and "Focus Gen 2"), Kalon HSV-2 ELISA ("Kalon"), Biokit HSV-2 Rapid Test ("Biokit"), and HerpeSelect Express Rapid HSV-2 ("Express") against the Western blot test ("WB") as the "gold standard". Sensitivity and specificity of tests in this population with a high prevalence of HSV-2 (58% by WB) were: Focus Gen 1: 98.6% and 63.5%; Focus Gen 2: 99.3% and 52.3%; Biokit: 66% and 90.9%; Express: 99.3% and 44.3% and Kalon: 98.6% and 85.7%. Increasing the positive cut-off value improved the specificity of the Focus Gen 2-84.9% and Kalon to 92.2%. Focus Gen 2 offered no improvement in specificity over that of Focus Gen 1. Neither rapid assay could be recommended as either a stand-alone assay or as a confirmatory test. The results of Kalon using a cut-off of 1.5 were the most concordant with those of WB for all the approaches tested. However, low positive Kalon test results should be interpreted with caution as they could reflect early seroconversion or false positive results. © 2009 Elsevier B.V.
adult; antibody detection; article; cost effectiveness analysis; diagnostic accuracy; enzyme linked immunosorbent assay; fisherman; Herpes simplex virus 2; human; intermethod comparison; Kenya; male; nonhuman; priority journal; sensitivity and specificity; seroconversion; serology; Western blotting; Adolescent; Adult; Antibodies, Viral; Herpes Genitalis; Herpesvirus 2, Human; Humans; Kenya; Male; Sensitivity and Specificity; Serologic Tests; Young Adult; Human herpesvirus 2
