Departments of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia; Departments of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia; Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
Tesfaye, A., Departments of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia; Mehari, T., Departments of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia; Ashenafi, M., Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
From 143 lactic acid bacteria (LAB) recovered from locally fermented products, 27 strains were antagonistic against sensitive LAB indicator (Lab. brevis 3). Based on in vitro (acid-bile tolerance and antagonism against foodborne pathogens) criteria 9 LAB were selected for in vivo mice model infection test LAB (pure or mixed cultures) and Salmonella Typhimurium DT104 were administered orally to mice at log 6 and log 4 cfu/ml, respectively. The count of Salmonella Typhimurium DT104 from the frces of co-infected mice was significantly reduced (p<0.01) by mixed LAB culture (MLC) 3, MLC2, MLC 1, Lab. plantarum 1, Ped pentosaceus 1, Lab. delbreuckiissp delbreuckii, Lab. brevis 1, Lab. acidophilus 1 and Lab. plantarum 2. MLC 3 and 2 totally eliminated the test pathogen from the frces co-infected mice at day 15 and 16, respectively. The effects of MLCs were found better than single LAB cultures. Besides, the effect of single LAB cultures was improved much at log 9.00 cful ml administrations. Both the in vitro and in vivo probiotic analyses results strongly suggest that LAB, most single and all MLCs are possible candidate probiotics that can be used for the formulation of starters to prepare safe and bioprotective products. Copyright © 2011 by New Century Health Publishers, LLC.
probiotic agent; animal experiment; animal model; article; bacterium culture; bacterium isolate; controlled study; drug safety; fermentation; Lactobacillus; Lactobacillus acidophilus; Lactobacillus curvatus; Lactobacillus delbrueckii; Lactobacillus fermentum; Lactobacillus paracasei; Lactobacillus plantarum; mouse; nonhuman; Pediococcus pentosaceus; pH measurement; Salmonella typhimurium; salmonellosis
