Babalola S.A., Suleiman M.M., Hassan A.Z., Adawa D.
Department of Veterinary Surgery and Theriogenology, Michael Okpara University of Agriculture, Umudike, Nigeria; Department of Pharmacology and Toxicology, Michael Okpara University of Agriculture, Umudike, Nigeria; Department of Veterinary Surgery and Ra
Babalola, S.A., Department of Veterinary Surgery and Theriogenology, Michael Okpara University of Agriculture, Umudike, Nigeria; Suleiman, M.M., Department of Pharmacology and Toxicology, Michael Okpara University of Agriculture, Umudike, Nigeria; Hassan, A.Z., Department of Veterinary Surgery and Radiology, Michael Okpara University of Agriculture, Umudike, Nigeria; Adawa, D., Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Nigeria
This study evaluates the methanolic crude extract of Datura metel L. seeds (family Solanacease) as a potential oral anaesthetic in dogs. Following the oral acute toxicity study to determine the median lethal dose (LD50) of the extract in twelve (12) mice, the extract was relatively safe since when administered at the highest dose (5,000 mg/kg) no sign of toxicity and no death was recorded. In the in vivo sedative and anaesthetic study of the effect of Datura metel L in this work, the observed pharmacological effect of the extract administered orally to five (5) dogs separately at a dose rate of 0.6, 1.2, 1.5, 2 and 2.4 g/kg respectively during establishment of a suitable pilot oral anaesthetic doses, showed a graded dose response relationship. The extract at an oral dose of 2.4 g/kg induced surgical anaesthesia in dogs with increased heart and respiratory rates (107 to 205 bpm and 36.33 to 41.33 cpm) respectively, normal rectal temperature (37.83°C), adequate tissue perfusion, good muscle relaxation but poor analgesia, loss of anal sphincter tone and loss of pupillary reflex. The dogs recovered without any complications. This study has shown that the seed extract of Datura metel L. is relatively safe, induced sleep similar to that of thiopentone sodium anaesthesia with good anaesthetic indices at the oral dose rate of 2.4 g/kg in dogs. © IDOSI Publications, 2014.
alkaloid; anesthetic agent; Datura metel extract; flavonoid; glycoside; methanol; plant extract; resin; tannin derivative; terpenoid derivative; unclassified drug; acute toxicity; animal experiment; anus sphincter; article; breathing rate; controlled study; Datura metel; dog; dose response; drug isolation; drug safety; drug screening; heart rate; mouse; muscle relaxation; nonhuman; plant seed; pupil reflex; rectum temperature; sedation; sleep induction; tissue perfusion
