Tom Ashafa A.O., Yakubu M.T., Grierson D.S., Afolayan A.J.
Evaluation of aqueous extract of Felicia muricata leaves for anti-inflammatory, antinociceptive, and antipyretic activities
Department of Botany, University of Fort Hare, Alice 5700, South Africa
Tom Ashafa, A.O., Department of Botany, University of Fort Hare, Alice 5700, South Africa; Yakubu, M.T., Department of Botany, University of Fort Hare, Alice 5700, South Africa; Grierson, D.S., Department of Botany, University of Fort Hare, Alice 5700, South Africa; Afolayan, A.J., Department of Botany, University of Fort Hare, Alice 5700, South Africa
Context: Felicia muricata Thunb. (Nees) (Asteraceae) leaves are used in folklore medicine of South Africa as an oral remedy for pain and inflammation. However, the efficacy of the plant part is yet to be validated with scientific experiments. Objective: The current study is an effort to investigate the anti-inflammatory, antinociceptive, and antipyretic activities of aqueous extract of F. muricata leaves. Materials and methods: The phytochemical screening of aqueous extract of Felicia muricata leaves and the efficacy of the extract at the doses of 50, 100, and 200mg/kg body weight was investigated in experimental animals using several models of inflammation (paw edema induced by carrageenan and egg albumin), nociception (acetic acid-induced writhing, formalin-induced pain and tail immersion), and fever (brewer's yeast-induced hyperthermia). Results: The extract contained alkaloids, flavonoids, tannins, saponins, and phenolics. The extract dose-dependently reduced (P <0.05) the number of writhes and stretches induced by acetic acid, number of licks induced by formalin, paw volumes induced by carrageenan and egg albumin. The reaction time by the tail of the extract-treated animals to the hot water also increased. The extract also reduced hyperthermia induced by brewer's yeast. The highest dose (200mg/kg body weight of the extract) produced the best result in all cases. Discussion and conclusion: This study revealed that the aqueous extract of Felicia muricata leaves possessed anti-inflammatory, antinociceptive and antipyretic activities. These findings have therefore supported the use of aqueous extract of Felicia muricata leaves in the traditional medicine of South Africa as an oral remedy for pains, inflammation, and fever. © 2010 Informa Healthcare USA, Inc.
alkaloid; Felicia muricata extract; flavonoid; indometacin; morphine; phenol derivative; plant extract; saponin; tannin derivative; unclassified drug; water; animal experiment; antiinflammatory activity; antinociception; antipyretic activity; article; Asteraceae; chemical composition; controlled study; dose response; drug efficacy; drug response; Felicia muricata; fever; hyperthermia; inflammation; male; nonhuman; pain; paw edema; plant leaf; rat; Analgesics, Non-Narcotic; Animals; Anti-Inflammatory Agents, Non-Steroidal; Antipyretics; Asteraceae; Dose-Response Relationship, Drug; Drug Discovery; Drug Evaluation, Preclinical; Edema; Fever; Male; Medicine, African Traditional; Mice; Pain Measurement; Phytotherapy; Plant Extracts; Plant Leaves; Rats; Rats, Wistar; Time Factors; Animalia; Asteraceae; Felicia muricata; Saccharomyces cerevisiae
